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[113] Lab Chip, 2021, Accepted, https://doi.org/10.1039/D1LC00485A

The role of liquid biopsies in prostate cancer management

Chi-Ju Kim+, Liang Dong+, Sarah Amend*, Yoon-Kyoung Cho* and Kenneth Pienta*

The need to appropriately diagnose and guide the serial treatment of men with prostate cancer has led to the implementation of many studies to apply liquid biopsies to prostate cancer management. This review describes recent advancements in isolation and detection technology and the strength and weaknesses of the three circulating biomarkers. The clinical studies based on liquid biopsy results are summarized to depict the future perspective in the role of liquid biopsy on prostate cancer management.

This article is part of the themed collection: Microfluidics for hematology

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[112] J. Am. Chem. Soc. 2021, 143, 28, 10582–10589

Atomically Conformal Metal Laminations on Plasmonic Nanocrystals for Efficient Catalysis

Anubhab Acharya, Sateesh Dubbu, Sumit Kumar, Nitee Kumari, Yeseul Kim, Sunae So, Taewan Kwon, Zhipeng Wang, Junbeom Park, Yoon-Kyoung Cho, Junsuk Rho, Sang Ho Oh, Amit Kumar, In Su Lee

We introduce a “confine and shine” strategy to homogeneously modify the different surface curvatures of plasmonic NCs with ultrathin conformal layers of diverse catalytic noble metals. This self-limited epitaxial skinlike metal growth harvests the localized surface plasmon resonance to induce reduction chemistry directly on the NC surface, confined inside hollow silica. Unlike the conventional thick, anisotropic, and dendritic shells, which show severe nonradiative damping, the skinlike metal lamination preserves the key plasmonic properties of the core NCs. Consequently, the plasmonic–catalytic hybrid nanoreactors can carry out a variety of organic reactions with impressive rates.

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[111] ACS Appl. Mater. Interfaces 2021, 13, 25, 29313–29324

Glycolipid-Anchored Proteins on Bioengineered Extracellular Vesicles for Lipopolysaccharide Neutralization

Divakara SSM Uppu, Yoohong Min, Inun Kim, Sumit Kumar, Juhee Park, and Yoon-Kyoung Cho*

This study reports bioengineered EVs with CD14, a natural glycosylphosphatidylinositol (GPI)-anchored protein and a selectively enriched native membrane protein on EVs. We demonstrated that producer cells transfected with genes encoding for GPI-anchored and transmembrane glycoproteins selectively display the former over the latter on bioengineered EVs. Natural GPI-anchored proteins are conserved receptors for bacterial toxins; for example, CD14 is an innate immune receptor for lipopolysaccharide (LPS), a gram-negative bacterial endotoxin. We reported that unlike soluble CD14, bioengineered EVs harboring CD14 reduce (50–90%) LPS-induced cytokine responses in mouse macrophages, including primary cells, possibly by reduced cell surface binding of LPS. These findings highlight the importance of harnessing the native EV membrane proteins, like GPI-anchored proteins, for functional studies such as toxin neutralization.

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[110] Frontiers in Bioengineering and Biotechnology, 2021, 9, 358

New Multiscale Characterization Methodology for Effective Determination of Isolation–Structure–Function Relationship of Extracellular Vesicles

Thanh Huyen Phan, Shiva Kamini Divakarla, Jia Hao Yeo, Qingyu Lei, Priyanka Tharkar, Taisa Nogueira Pansani, Kathryn G Leslie, Maggie Tong, Victoria A Coleman, Åsa Jämting, Mar-Dean Du Plessis, Elizabeth J New, Bill Kalionis, Philip Demokritou, Hyun-Kyung Woo, Yoon-Kyoung Cho, Wojciech Chrzanowski

Here, using a range of optical and non-optical techniques, we showed that the differences in molecular composition of EVs isolated using two isolation methods correlated with the differences in their biological function. Our results demonstrated that the isolation method determines the composition of isolated EVs at single and sub-population levels. Besides the composition, we measured for the first time the dry mass and predicted sedimentation of EVs. These parameters were likely to contribute to the biological and functional effects of EVs on single cell and cell cultures.

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[109] ACS Applied Materials & Interfaces, 2021, 13, 13, 15837–15846

Spontaneous wrinkle formation on hydrogel surfaces using photoinitiator diffusion from oil-water interface

Eujin Um, Yoon-Kyoung Cho*, Joonwoo Jeong*

Patterning wrinkles on three-dimensional curved or enclosed surfaces can be challenging due to difficulties in application of uniform films and stresses on such structures. In this study, we demonstrate a simple one-step wrinkle-formation method on various hydrogel structures utilizing the oil-water interfaces. By diffusion of the photoinitiator from the oil phase to the prepolymer solution in water through the interface, a characteristic crosslinking gradient is set up in the hydrogel.

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[108] Science, 371, 6526, eabe8678, (2021)

Response to Comment on “Boosted molecular mobility during common chemical reactions”

Huan Wang, Myeonggon Park, Ruoyu Dong, Junyoung Kim, Yoon-Kyoung Cho, Tsvi Tlusty, Steve Granick*

Günther et al. report that their control experiment using randomized magnetic field gradient sequences disagreed with findings we had reported using linear gradients. However, we show that measurements in our laboratory are consistent using both methods.

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[107] ACS Applied Bio Materials, 2021, 4, 5, 3891–3908

Advances in Facemasks during the COVID-19 Pandemic Era

Mamata Karmacharya, Sumit Kumar, Oleksandra Gulenko, and Yoon-Kyoung Cho*

The outbreak of coronavirus disease (COVID-19) has transformed the daily lifestyles of people worldwide. COVID-19 was characterized as a pandemic owing to its global spread, and technologies based on engineered materials that help to reduce the spread of infections have been reported. Nanotechnology present in materials with enhanced physicochemical properties and versatile chemical functionalization offer numerous ways to combat the disease. Facemasks are a reliable preventive measure, although they are not 100% effective against viral infections. This study provides a comprehensive review on face masks, from the manufacturing stage to the practical application, and their ability to combat COVID-19. Further, we discuss the impacts of masks on the environment, while considering safe industrial production in the future. We also discuss the available options for the future research directions without negative environmental impacts.

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[106] Nano Letters, 2021, 21, 1, 279–287

Surface-Textured Mixed-Metal-Oxide Nanocrystals as Efficient Catalysts for ROS Production and Biofilm Eradication

Nitee Kumari, Sumit Kumar, Mamata Karmacharya, Sateesh Dubbu, Taewan Kwon, Varsha Singh, Keun Hwa Chae, Amit Kumar*, Yoon-Kyoung Cho*, and In Su Lee*

Existing antimicrobials cannot function in the complex and stressful chemical conditions found in biofilms, and as a result, they are unable to infiltrate, diffuse into, and eradicate the biofilm and its associated matrix. Here, we introduce mixed-FeCo-oxide-based surface-textured nanostructures (MTex) as highly efficient magneto-catalytic platforms. These systems can produce defensive ROS over a broad pH range and can effectively diffuse into the biofilm and kill the embedded bacteria.

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[105] Nano Letters, 2021, 21, 1, 337–343

Photoactive Antiviral Face Mask with Self-Sterilization and Reusability

Sumit Kumar, Mamata Karmacharya, Shalik Ram Joshi, Oleksandra Gulenko, Juhee Park, Gun-Ho Kim, and Yoon-Kyoung Cho*

Here, we applied a dual-channel spray-assisted nanocoating hybrid of shellac/copper nanoparticles (CuNPs) to a nonwoven surgical mask, thereby increasing the hydrophobicity of the surface and repelling aqueous droplets. The resulting surface showed outstanding photoactivity (combined photocatalytic and photothermal properties) for antimicrobial action, conferring reusability and self-sterilizing ability to the masks.

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[104] Biomaterials, 265, 120420, (2021)

Adaptive Architecture and Mechanoresponse of Epithelial Cells on a Torus

Sun-Min Yu, Bo Li, François Amblard, Steve Granick, Yoon-Kyoung Cho*

Curvature is a geometric feature widely observed in the epithelia and critical to the performance of fundamental biological functions. In this study, we revealed morphological adaptations and mechanobiological behaviors of epithelial cells on a torus surface with Gaussian curvatures. Our results collectively indicate the ability of epithelial cells to sense Gaussian curvatures and increase the expression of cytoplasmic and nucleus intermediate filaments as a protective measure against curvature-induced mechanical stress.

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[103] J. Extracell Vesicles. 10, e12044 (2020)

Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium

Liang Dong, Richard C. Zieren, Kengo Horie, Chi‐Ju Kim, Emily Mallick, Yuezhou Jing, Mingxiao Feng, Morgan D. Kuczler, Jordan Green, Sarah R. Amend, Kenneth W. Witwer, Theo M. de Reijke, Yoon‐Kyoung Cho, Kenneth J. Pienta, Wei Xue

One of the challenges that restricts the evolving extracellular vesicle (EV) research field is the lack of a consensus method for EV separation. This may also explain the diversity of the experimental results, as co‐separated soluble proteins and lipoproteins may impede the interpretation of experimental findings. In this study, we comprehensively evaluated the EV yields and sample purities of three most popular EV separation methods, ultracentrifugation, precipitation and size exclusion chromatography combined with ultrafiltration, along with a microfluidic tangential flow filtration device, Exodisc, in three commonly used biological samples, cell culture medium, human urine and plasma.

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[102] Cancers, 12, 10, 2822, (2020)

Detection of EGFR mutations using bronchial washing-derived
extracellular vesicles in patients with non-small-cell lung carcinoma

Juhee Park, Chaeeun Lee, Jung Seop Eom, Mi-Hyun Kim *, Yoon-Kyoung
Cho *

Here, we demonstrate the clinical potential of BW-derived EVs as a liquid-biopsy sample for prognosis and precision medicine in patients with lung cancer. The acquisition of T790M resistance mutation was detected earlier in BW-derived EVs than in plasma or tissue samples. The longitudinal analysis of BW-derived EVs showed excellent correlation with the disease progression measured by CT images.

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[101] ACS Nano, 14, 11, 14971–14988, (2020)

Three-Dimensional Human Liver-Chip Emulating Pre-Metastatic Niche Formation by Breast Cancer-Derived Extracellular Vesicles

Junyoung Kim, Chaeeun Lee, Inun Kim, Jooyoung Ro, Jungmin Kim, Yoohong Min, Juhee Park, Vijaya Sunkara, Yang-Seok Park, Issac Michael, Young-Ae Kim, Hee Jin Lee, Yoon-Kyoung Cho*

The 3D microfluidic human liver-chip developed in this study demonstrates how breast cancer-derived extracellular vesicles induce the formation of a pre-metastatic niche, promoting the adhesion of circulating tumor cells to a distant organ such as the liver, enabling the investigation of cancer metastasis and a wide range of potential pharmacological therapies.

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[100] Science, 369, 6503, 537-541, (2020)

Boosted molecular mobility during common chemical reactions

Huan Wang, Myeonggon Park, Ruoyu Dong, Junyoung Kim, Yoon-Kyoung Cho, Tsvi Tlusty, Steve Granick*

During a chemical reaction, the reorganization of solvent molecules not directly in contact with reactants and products is normally viewed as a simple diffusion response. Wang et al. studied molecular diffusion in six common reactions—including the copper-catalyzed click reaction and the Diels-Alder reaction—with pulsed-field gradient nuclear magnetic resonance. They observed a boost in mobility relative to Brownian diffusion that was stronger for the catalyzed reactions that were studied. The mobilities for the click reaction were verified with a microfluidic gradient method. They argue that energy release produces transient translational motion of reacting centers that mechanically perturbs solvent molecules.

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[99] Cells, 9, 6, 1434, (2020)

Mechanical Adaptations of Epithelial Cells on Various Protruded Convex Geometries

Sun-Min Yu+, Bo Li+, Steve Granick and Yoon-Kyoung Cho*

Here, we cultured cells on various protruded convex structures such as triangle, square, and circle shape fabricated using two-photon laser lithography and quantitatively analyzed individual cells. We found that intermediate filaments, vimentin, and cytokeratin 8/18, play important roles in the growth and adaptation of epithelial cells by enhancing expression level on convex structure depending on the shape. In addition, microtubule building blocks, α-tubulin, was also responded on geometric structure, which indicates that intermediate filaments and microtubules can cooperatively secure the mechanical stability of epithelial cells on the convex surface. Altogether, the current study will expand our understanding of mechanical adaptations of cells on out-of-plane geometry.

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[98] Nature Biomedical Engineering, 4, 591-600, (2020)

A fidget spinner for the point-of-care diagnosis of urinary tract infection

Issac Michael, Dongyoung Kim, Oleksandra Gulenko , Sumit Kumar, Saravana Kumar , Jothi Clara, Dong Yeob Ki, Juhee Park , Hyun Yong Jeong, Taek Soo Kim, Sunghoon Kwon  and Yoon-Kyoung Cho *

Here, we describe a custom-made fidget spinner that rapidly concentrates pathogens in 1-ml samples of undiluted urine by more than 100-fold for the on-device colorimetric detection of bacterial load and pathogen identification. The device enabled the on-site detection of infection with the naked eye within 50 min in urine samples from 39 patients suspected of having a urinary tract infection. We also show that, in 30 clinical samples of urinary tract infection, the device can be used to perform an antimicrobial susceptibility test for the antimicrobial drugs ciprofloxacin and cefazolin within 120 min. The fidget spinner could be used in low-resource settings as an inexpensive handheld point-of-care device for the rapid concentration and detection of pathogens in urine samples.

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[97] Diagnostics, 10, 4, 249, (2020)

Circulating Tumor Cells Enumerated by a Centrifugal Microfluidic Device as a Predictive Marker for Monitoring Ovarian Cancer Treatment: A Pilot Study

Hyera Kim , Minji Lim, Jin Young Kim, So-Jin Shin, Yoon-Kyoung Cho* and Chi Heum Cho*

This pilot study of 13 patients with ovarian cancer aimed to investigate a strategy for the enumeration and detection of CTCs based on a newly-developed centrifugal microfluidic device equipped with a fluid-assisted separation technology (FAST) disc and to demonstrate the correlations among CTC counts from the new device, CA125 concentrations, and clinical course of the disease.

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[96] Angewandte Chemie International Edition, 59, 4, (2020)

Nanocatalosomes as Plasmonic Bilayer Shells with Interlayer Catalytic Hot Nanospaces for Solar‐Light‐Induced Reactions

Amit Kumar, Nitee Kumari, Sateesh Dubbu, Sumit Kumar, Taewan Kwon, Jung Hun Koo, Jongwon Lim, Inki Kim, Yoon-Kyoung Cho, Junsuk Rho, and In Su Lee*​

Paramount interest and challenges remain in designing and synthesizing catalysts with nature‐like complexities at few‐nm scale to harness unprecedented functionalities by using sustainable solar light. We introduce “nanocatalosomes”— bio‐inspired bilayer‐vesicular design of nanoreactor with metallic bilayer hollow shell‐in‐shell structure, having numerous controllable confined cavities within few‐nm interlayer space, customizable with different noble metals. This work paves the way towards next‐generation nanoreactors, craftable at few‐nm scale to maximize their functionality and customizable to carry out diverse chemical transformations efficiently with green solar energy.

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[95] Anaytical Chemistry, 92, 8, 6010-6018, (2020)

EV-Ident: Identifying tumor-specific extracellular vesicles by size fractionation and single-vesicle analysis

Dongyoung Kim+, Hyun-Kyung Woo+, Chaeeun Lee, Yoohong Min, Sumit Kumar, Vijaya Sunkara, Hwi-Gyeong Jo, Young Joo Lee, Jisun Kim, Hong Koo Ha, and Yoon-Kyoung Cho*

Here, we introduce EV-Ident for preparation of subpopulations of EVs in three different size fractions: large EVs (EV200 nm; 200−1,000 nm), medium EVs (EV100 nm; 100−200 nm), and small EVs (EV20 nm; 20−100 nm). As a proof-of-concept, we analyzed the presence of human epidermal growth factor receptor 2 (HER2) and prostate-specific membrane antigen (PSMA) in breast cancer and prostate cancer cell-derived EVs, respectively, using three different size fractions at the single-EV level. By reducing the complexity of EV heterogeneity in each size fraction, we found that HER2-positive breast cancer cells showed the greatest expression of HER2 in EV20 nm, whereas PSMA expression was the highest in EV200 nm derived from PSMA expressing prostate cancer cells. This increase in HER2 expression in EV20 nm and PSMA expression in EV200 nm was further confirmed in plasma-derived nanoparticles (PNPs) obtained from breast and prostate cancer patients, respectively.

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[94] Nature Nanotechnology, 15, 4, 1-11, (2020)

Targeted crystallization of mixed-charge nanoparticles in lysosomes induces selective death of cancer cells

Magdalena Borkowska, Marta Siek, Diana V. Kolygina, Yaroslav I. Sobolev, Slawomir Lach, Sumit Kumar, Yoon-Kyoung Cho, Kristiana Kandere-Grzybowska & Bartosz A. Grzybowski

Here, we show that mixed-charge nanoparticles covered with certain ratios of positively and negatively charged ligands can selectively target lysosomes in cancerous cells while exhibiting only marginal cytotoxicity towards normal cells. This selectivity results from distinct pH-dependent aggregation events, starting from the formation of small, endocytosis-prone clusters at cell surfaces and ending with the formation of large and well-ordered nanoparticle assemblies and crystals inside cancer lysosomes. These assemblies cannot be cleared by exocytosis and cause lysosome swelling, which gradually disrupts the integrity of lysosomal membranes, ultimately impairing lysosomal functions and triggering cell death.

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