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Dehydrated Aqueous Two-Phase System Micro-domain Retain Shape upon Rehydration to Allow Patterned Reagent Delivery to Cells
C´edric Bathany, Juhee Park, Yoon-Kyoung Cho* and Shuichi Takayama*
Aqueous reagent solution micro-domains with sharp boundaries and defined shapes are created over cell monolayers within an immiscible bulk aqueous phase through rehydration of freestanding and portable dried reagent patches of the corresponding shape....
[43] Journal of Colloid and Interface Science, 411, 1, 122-128, (2013)
"Aminosilane layers on the plasma activated thermoplastics: influence of solvent on its structure and morphology"
Vijaya Sunkara and Yoon-Kyoung Cho*
The molecular mechanism of the interaction between silane, e.g. APTES, with the plasma activated thermoplastic surfaces was investigated. The effect of the type of solvent during the silane deposition step on the thickness, surface morphology, stability and chemical properties of the APTES layer on plasma treated thermoplastics was explored.
[42] Scientific Reports, 3, 1, 2255, (2013)
Make Caffeine Visible: a Fluorescent Caffeine “Traffic Light” Detector
Wang Xu, Tae-Hyeong Kim, Duanting Zhai, Jun Cheng Er, Liyun Zhang, Anup Atul Kale, Bikram Keshari Agrawalla, Yoon-Kyoung Cho* and Young-Tae Chang*
We report a novel aqueous phase fluorescent caffeine sensor named Caffeine Orange which exhibits 250-fold fluorescence enhancement upon caffeine activation and high selectivity. To utilize this probe in real life, we developed a microfluidic device to make caffeine detection quick, sensitive and automated.
[41] Electrophoresis, 35, 2-3, 245-257, (2013)
Pumps for microfluidic cell cultures
Chang Kyu Byun, Kameel Abi-Samra, Yoon-Kyoung Cho*and Shuichi Takayama*
In this review, we summarize recent advances in pumping techniques for microfluidic cell culture and discuss their advantages and possible drawbacks.
[40] Lab Chip, 13, 18, 3747-3754, (2013)
Flow-enhanced Electrochemical Immunosensors on Centrifugal Microfluidic Platforms
Tae-Hyeong Kim+, Kameel Abi-Samra+, Vijaya Sunkara, Dong-Kyu Park, Mary Amasia, Nahui Kim, Jintae Kim, Hanshin Kim, Marc Madou* and Yoon-Kyoung Cho*
We present a novel fullyintegrated centrifugal microfluidic devicewith features for target antigen capture from biological samples, via a bead-based enzyme-linkedimmune-sorbent assay, and flow-enhanced electrochemicaldetection.
[39] Lab Chip, 13, 16, 3253-3260, (2013)
Electrochemical velocimetry on centrifugal microfluidic platforms
Kameel Abi-Samra+, Tae-Hyeong Kim+, Dong-Kyu Park, Nahui Kim, Jintae Kim, Hanshin Kim, Yoon-Kyoung Cho,* and Marc Madou*
We demonstrate that electrochemical velocimetry is a straightforward technique to accurately monitor flow on rotating microfluidic platforms in real-time.
[38] Anal. Chem., 85, 5, 2954–2960, (2013)
Lab-on-a-Disc for Simultaneous Determination of Nutrients in Water
Hyundoo Hwang, Yubin Kim, Juhye Cho, Ji-yoon Lee, Man-Sik Choi*, and Yoon-Kyoung Cho*
We introduce a new fully integrated centrifugal microfluidic device for simultaneous determination of five major inorganic nutrients: nitrite, nitrate+nitrite, ammonium, orthophosphate, and silicate.
[37] JACS, 135, 6, 2242–2247, (2013)
Productive Chemical Interaction Between a Bacterial Micro-colony Couple is Enhanced by Periodic Relocation
Chang Kyu Byun, Hyundoo Hwang, Woon Sun Choi, Toshiyuki Yaguchi, Jiwoon Park, Dasol Kim, Robert J. Mitchell*, Taesung Kim*, Yoon-Kyoung Cho*and Shuichi Takayama*
This paper describes a system to study how small physical perturbations can affect bacterial community behavior in unexpected ways through modulation of diffusion and convective transport of chemical communication molecules and resources
[36] Sensors & Actuators: B. Chemical, 178, 648-655, (2013)
Geometry Effects on Blood Separation Rate on a Rotating Disc
Tae-Hyeong Kim, Hyundoo Hwang, Robert Gorkin, Marc Madou, Yoon-Kyoung Cho
The geometric factors for faster particle separation rate on a centrifugal microfluidic platform are described
[35] Biomedical Microdevices, 15, 4, 627-634, (2013)
Three dimensional multicellular co-cultures and anti-cancer drug assays in rapid prototyped multilevel microfluidic devices
Hyundoo Hwang, Juhee Park, Changsik Shin, YoonKyung Do, Yoon-Kyoung Cho*
This report presents a multilevel microfluidic platform for robust construction of hydrogel scaffold in microchannels and its application to three dimensional (3D) multicellular co-cultures and assays.
[34] ACS Appl. Mater. Interfaces, 4, 12, 6537-6544, (2012)
Investigation on the Mechanism of Aminosilane-Mediated Bonding of Thermoplastics and Poly(dimethylsiloxane)
Vijaya Sunkara and Yoon-Kyoung Cho*
A possible mechanism for the aminosilane-mediated room-temperature (RT) bonding of thermoplastics and poly(dimethylsiloxane) (PDMS) is presented.
[32] ACS Appl. Mater. Interfaces, 4, 12, 6537-6544, (2012)
Aqueous two-phase system-derived biofilms for bacterial interaction studies
Toshiyuki Yaguchi, Mohammed Dwidar, Chang Kyu Byun, Brendan Leung, Siseon Lee,
Yoon-Kyoung Cho, Robert J. Mitchell,* and Shuichi Takayama*
We describe patterning of bacterial biofilms using polymer-based aqueous two-phase system (ATPS) microprinting protocols.
[31] Anal. Chem., 84, 5, 2133–2140, (2012)
Lab-on-a-Disc for Fully Integrated Multiplexed Immunoassay
Jiwoon Park,Vijaya Sunkara, Tae-Hyeong Kim, Hyundoo Hwang and Yoon-Kyoung Cho*
This paper presents a cost-effective, rapid, and fully automated lab-on-a-disc for simultaneous detection of multiple protein biomarkers in raw samples such as whole blood or whole saliva.
[30] ACS Appl. Mater. Interfaces, 4, 1, 34–39, (2012)
Increased Graphitization in Electrospun Single Suspended Carbon Nanowires Integrated with Carbon-MEMS and Carbon-NEMS Platforms
Swati Sharma, Ashutosh Sharma*, Yoon-Kyoung Cho, and Marc Madou*
Single suspended carbon nanowires (CNWs) integrated on carbon-MEMS (CMEMS) structures are fabricated by electrospinning of SU-8 photoresist followed by pyrolysis.
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