This protocol demonstrates how to achieve femto molar detection sensitivity of proteins in 10 µL of whole blood within 30 min. This can be achieved by using electrospun nanofibrous mats integrated in a lab-on-a-disc, which offers high surface area as well as effective mixing and washing for enhanced signal-to-noise ratio.

[56] Immunology Letters, 172, 21–28 (2016)

• Pre-Tfh cells are found proximal to CD8α− DCs in the MZ bridging channels.

• The role of IL-6 secreted from CD8α− DCs is minor in inducing Tfh cells.

• CD8α− DCs dominantly induce Tfh cells over other APCs.

[55] Micromachines 7(2), 32 (2016)

By identifying the finest difference between the point-of-care testing and extreme point-of-care infrastructure, this review captures the evolving diagnostic needs of developing countries paired with infrastructural challenges with technological hurdles to healthcare delivery in extreme point-of-care settings. In particular, the requirements for making centrifugal diagnostic devices viable in developing countries are discussed based on a detailed analysis of the demands in different clinical settings including the distinctive needs of extreme point-of-care settings.

[54] Analyst, 141, 371-381 (2016)

We present an overview of the current EV isolation and characterization methods in addition to their applications and limitations. Furthermore, EVs as the potential emerging biomarkers in cancer management and their clinical implementation are briefly discussed.

 

[53] Anal.Chem. 87 (15), 7865–7871 (2015)

We have developed a fully integrated centrifugal microfluidic device for rapid on-site quantification of lipids from microalgal samples. The fully automated serial process involving cell sedimentation and lysis, liquid-liquid extraction, and colorimetric detection of lipid contents was accomplished within 13 min using a lab-on-a-disc.

[52] Biosensors & Bioelectronics, 70, 115–121 (2015)

Chronoamperometric recordings of a solution in movement mediated by a paper wicking are converted into a given signature hydrodynamic conditions of the porous material. The electrochemical sensor is used to screen porous material characteristics and test fluid flow modifiers potency.

 

[51] Lab Chip, 15, 478-485 (2015)

We present a highly efficient lab-on-a-disc composed of TiO2nanofibrous mat for sensitive detection of serum proteins with a broad dynamic range, with only 10 μL of whole blood within 30 min. The TiO2 nanofibers provide high specific surface area as well as active functional groups to capture large amounts of antibodies on the surface.

[50] Analyst, 140, 616-622 (2015)

In this paper, we demonstrated significantly enhanced antibacterial activity of hierarchical anatase TiO2 NFs against Staphylococcus aureus in the presence of UV light. Furthermore, the uniform deposition of a large quantity of Ag nanoparticles on the surface of the TiO2 NFs ensured a significant enhancement of the antibacterial performance, even under dark conditions. 

[49] Analyst, 140, 113-126 (2015)

We report the surface modification of thermoplastic nanochannels and an assessment of the associated surface charge density, zeta potential and electroosmotic flow (EOF). Carboxylated PMMA nanochannels were efficient for the transport and elongation of λ-DNA while these same DNA molecules were unable to translocate through aminated nanochannels with lower surface charge density and zeta potential.

[48] Anal. Chem., 86(22) 11349-11356, (2014)

In this paper, we propose a centrifugal-force-based size-selective CTC isolation platform that can isolate and enumerate CTCs from whole blood within 30 s with high purity. Overall, our CTC isolation device enables rapid and label-free isolation of CTCs with high purity, which should greatly improve downstream molecular analyses of captured CTCs.

[47] ACS Appl. Mater. Interfaces., 6, 12189−12195 (2014)

This paper demonstrates  the first semiconductor-based nanofiber sensor suspended on carbon electrodes that has achieved by a relatively simple and cost-effective electrospinning method. Furthermore, these sensors demonstrate a high sensitivity, as well as a stable ohmic contact, due to the large surface area of the TiO2 NWs and the carbon-carbon contact between the suspended TiO2/C NFs and carbon electrodes. 

 

[46] Anal. Chem., 86 (8) 3841–3848 (2014)

This paper demonstrates that molecular detection of food-borne pathogens can be integrated into a single, compact device with great potential as a point of care diagnostic tool. Three main functions, DNA extraction, isothermal recombinase polymerase amplification (RPA), and detection, are integrated on a single device for the molecular detection of Salmonella.

 

[45] Integr. Biol., 6, 267-276 (2014)

Roles of small GTPases in lysophosphatidic acid-induced chemotaxis of SKOV3 ovarian cancer cells were first investigated using a novel microfluidic assay platform. Real-time tracking of individual cells in the rapid-prototyped multilevel microfluidic devices provided us substantial quantitative information about the chemotaxis of the cells under a linear and stable concentration gradient. This integrative study of cellular chemotaxis would provide us new biological insights, which could not be obtained through the traditional tools for cell migration assays.

 

[44] Journal of MaterialsChemistry B. 1, 6020-6026 (2013)

Aqueous reagent solution micro-domains with sharp boundaries and defined shapes are created over cell monolayers within an immiscible bulk aqueous phase through rehydration of freestanding and portable dried reagent patches of the corresponding shape....

[43] Journal of Colloid and Interface Science, 411, 1, 122-128 (2013)

The molecular mechanism of the interaction between silane, e.g. APTES, with the plasma activated thermoplastic surfaces was investigated. The effect of the type of solvent during the silane deposition step on the thickness, surface morphology, stability and chemical properties of the APTES layer on plasma treated thermoplastics was explored.

[42] Scientific Reports, 3, 2255 (2013)

We report a novel aqueous phase fluorescent caffeine sensor named Caffeine Orange which exhibits 250-fold fluorescence enhancement upon caffeine activation and high selectivity. To utilize this probe in real life, we developed a microfluidic device to make caffeine detection quick, sensitive and automated.

 

[41] Electrophoresis, 35, 245-257 (2013)

In this review, we summarize recent advances in pumping techniques for microfluidic cell culture and discuss their advantages and possible drawbacks.

 

[40] Lab Chip, 13, 3747-3754 (2013)

We present a novel fullyintegrated centrifugal microfluidic devicewith features for target antigen capture from biological samples, via a bead-based enzyme-linkedimmune-sorbent assay, and flow-enhanced electrochemicaldetection.

[39] Lab Chip, 13, 3253-3260 (2013)

We demonstrate that electrochemical velocimetry is a straightforward technique to accurately monitor flow on rotating microfluidic platforms in real-time.

[38] Anal. Chem., 85 (5), 2954–2960 (2013)

We introduce a new fully integrated centrifugal microfluidic device for simultaneous determination of five major inorganic nutrients: nitrite, nitrate+nitrite, ammonium, orthophosphate, and silicate.

[37] JACS, 135, 6, 2242–2247 (2013)

This paper describes a system to study how small physical perturbations can affect bacterial community behavior in unexpected ways through modulation of diffusion and convective transport of chemical communication molecules and resources

[36] Sensors & Actuators: B. Chemical, 178, 648-655 (2013)

The geometric factors for faster particle separation rate on a centrifugal microfluidic platform are described

 

[35] Biomedical Microdevices, 15, 4, 627-634 (2013)

This report presents a multilevel microfluidic platform for robust construction of hydrogel scaffold in microchannels and its application to three dimensional (3D) multicellular co-cultures and assays.

[34] ACS Appl. Mater. Interfaces, 4(12) 6537-6544 (2012)

A possible mechanism for the aminosilane-mediated room-temperature (RT) bonding of thermoplastics and poly(dimethylsiloxane) (PDMS) is presented.

[33] RSC Advances, 2, 9066-9070 (2012)

We report a versatile method for bonding dissimilar materials which is an important issue in the fabrication of micro- and nanofluidic devices.

[32] ACS Appl. Mater. Interfaces, 4(12) 6537-6544 (2012)

We describe patterning of bacterial biofilms using polymer-based aqueous two-phase system (ATPS) microprinting protocols.

[31] Anal. Chem., 84 (5), 2133–2140 (2012)

This paper presents a cost-effective, rapid, and fully automated lab-on-a-disc for simultaneous detection of multiple protein biomarkers in raw samples such as whole blood or whole saliva.

[30] ACS Appl. Mater. Interfaces, 4 (1), 34–39 (2012)

Single suspended carbon nanowires (CNWs) integrated on carbon-MEMS (CMEMS) structures are fabricated by electrospinning of SU-8 photoresist followed by pyrolysis.

[29] Carbon, 50(5), 1753–1761 (2012)

Single suspended carbon nanofibers on carbon micro-structures were fabricated by direct electrospinning and subsequent pyrolysis at 900 °C of carbon nanotube/polyacrylonitrile (CNT/PAN) composite material.

[28] Lab Chip, 11, 3404-3406 (2011)

This paper describes the active control of the capillary-driven flow in paper using a centrifugal device.

[27] Lab Chip, 11, 2518-2525 (2011)

In situ measurements of the enhanced SERS signal are demonstrated by using dynamic SERS active sites resulted from optically induced electrokinetic mechanisms.

[26] Microfluidics and Nanofluidics, 11(5), 643-652 (2011)

we present a novel pumping technique for the centrifugal microfluidic disc platform, termed the thermo-pneumatic pump (TPP), that enables fluids to be transferred the center of a rotating disc by the thermal expansion of air.

[25] Lab Chip, 11 (8), 1434 - 1436 (2011)

We present elastomeric membrane valves integrated into a centrifugal microfluidic platform for precise control of fluid on a disc.

[24] Chem. Soc. Rev. 40, 3677-3702, (2011)

The fabrication of nanochannels in thermoplastics and elastomers is critically reviewed along with their potential applications.

[23] Lab Chip, 11, 962-965 (2011)

We report a simple, versatile, instantaneous and irreversible method for bonding thermoplastics to elastomeric polydimethylsiloxane (PDMS) at room temperature.

[22] Lab Chip, 1170-78 (2011)

We report a fully integrated lab-on-a-disc system for simultaneous analysis of clinical chemistry and enzyme-linked immuno-sorbent assay (ELISA) from whole blood.

 

[21] Lab Chip, 10, 3255-3264 (2010)

Distinct elastic interface of surface microfluidics enables novel three-dimensional (deformable) and time-dependent (capacitive) operations of the surface flow.

[20] J. Micromech. Microeng., 065010, (2010)

We report the on-chip concentration of bacteria using a dielectrophoretic (DEP) chip with 3D electrodes and subsequent laser-based DNA extraction in the same chip.

[19] Lab Chip, 1758-1773, (2010)

This paper presents an in-depth review of the centrifugal microfluidic platform for biomedical applications, while highlighting recent progress in the field and outlining the potential for future applications.

[18] Annals of Biomedical Engineering, 38, 2056–2067 (2010)

Microfabrication technologies have a high potential for novel approaches to access living cells at a cellular or even at a molecular level.

[17] Electrophoresis, 30, 3153–3159 (2009)

We report an insulator-based (or, electrodeless) dielectrophoresis utilizing microfabricated plastic membranes.

[16] Lab Chip, 9, 1548-1555 (2009)

We report a disc-based and fully automated microfluidic enzyme-linked immuno-sorbent assay (ELISA) system for the quantification of proteins from whole blood.

[15] Lab Chip, 7, 565-573 (2007)

We report a fully integrated, pathogen-specific DNA extraction CD platform utilizing innovative laser irradiated ferrowax microvalves (LIFM) and the rapid cell lysis method using target separation and laser-irradiated magnetic bead system (TS-LIMBS).

[14] Lab Chip, 7, 557-564, (2007)

We report a novel phase change based microvalve for rapid and versatile operation of multiple microvalves using nanoheaters, 10 nm-sized iron oxide nanoparticles dispersed in paraffin wax, and laser irradiation.

[13] Colloids and Surfaces A: Physicochem. Eng. Aspects, 294, 228-235 (2006)

[12] Microsystem Technologies, 12, 238-246 (2006)

[11] Biosensors and Bioelectronics, 21, 2161-2169, (2006)

[10] Lab Chip, 8, 845-850, (2005)

[09] J. Micromech. Microeng. 15(3), 455-462 (2005)

[08] Journal of Colloid Interface and Science, 278, 44-52 (2004 )

[07] J. Chemical Physics, 119,1,547-554 (2003)

[06] Langmuir, 17, 7732-7734 (2001)

[05] J. Chem. Phys, 110, 9688-9696 (1999)

[04] J. of Polymer Science: Part B: Polymer Physics, 35, 2961-2968 (1997)

[03] Tribology Intl., 30, 12, 889-894 (1997)

[02] Wear, 200, 346-352 (1996)

[01] J. Membrane Science, 104, 3, 219-230 (1995)

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