[95]“TGFβ1 in Breast Tumor-Derived Extracellular Vesicles Promotes the Adhesion of Circulating Tumor Cells on 3D Human Liver Chip”, Junyoung Kim, Chaeeun Lee, Inun Kim, Yoohong Min, Vijaya Sunkara, Juhee Park, Yang-Seok Park, Issac. J. Michael, Jooyoung Ro, Yoon-Kyoung Cho*, submitted


[94] "Near-field electrospinning for 3D stacked nanoarchitectures with high aspect ratio”, Yang-Seok Park, Jung Min Oh, Junyoung Kim, Seungyoung Park, Seungse Cho, Hyunhyub Ko, Yoon-Kyoung Cho*, submitted


[93] "Circulating tumor cells enumerated by a centrifugal microfluidic device as a predictive marker for treatment monitoring of ovarian cancer patients", Hyera Kim‡, Minji Lim‡, Jin Young Kim, So-Jin Shin, Yoon-Kyoung Cho*, Chi Heum Cho*, submitted


[92] "Highly Sensitive Detection of Hydrazine on Poly(Tannic Acid)-Coated Disposable Carbon Electrode" Monsur Jiaul Haque, Sumit Kumar, Jonathan Sabaté del Río, and Yoon-Kyoung Cho*, submitted


[91] "ExoFind: Identifying tumor specific extracellular vesicles by size fractionation and single-vesicle analysis", Dongyoung Kim‡, Hyun-Kyung Woo‡, Chaeeun Lee, Yoohong Min, Hong Koo Ha, and Yoon-Kyoung Cho*, submitted


[90] "AI-powered transmitted light microscopy for functional analysis of live cells", Dongyoung Kim, Yoohong Min, Jung Min Oh, and Yoon-Kyoung Cho*, submitted

[89] "​Dx-Fidget Spinner: A Pocket-Sized, Low-Cost, Hand-Powered Centrifuge for Point-of-Care Diagnostics of Infectious Diseases" Issac Michael, Dongyoung Kim, Oleksandra Gulenko, Saravana Kumar, Jothi Clara, Sumit Kumar, Dong-Yeob Ki, Juhee Park, Hyun Yong Jeong, Taek Soo Kim, Sunghoon Kwon, and Yoon-Kyoung Cho*,  Nature Biomedical Engineering, 2019, accepted

The possibility of functional roles played by platelets in close alliance with cancer cells has inspired the design of new biomimetic systems that exploit platelet–cancer cell interactions. Here, the role of platelets in cancer diagnostics is leveraged to design a microfluidic platform capable of detecting cancer‐derived extracellular vesicles (EVs) from ultrasmall volumes (1 µL) of human plasma samples. Further, the captured EVs are counted by direct optical coding of plasmonic nanoprobes modified with EV‐specific antibodies. Owing to the diverse function proteins associated with human platelets that can bind to the wide spectrum of cancer cell-derived extracellular vesicles, the resulting microdevice is capable for sensitive and accurate detection of cancer from ultra-small volumes of patient’s plasma samples.

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Effects of poly(ethylene glycol) on the wetting behavior and director configuration of lyotropic chromonic liquid crystals confined in cylinders

We investigate the effects of poly(ethylene glycol) (PEG) doping on nematic lyotropic chromonic liquid crystals (LCLCs) confined in a cylindrical cavity. We also confirm that the grafting of PEG to bare glass surfaces changes them from nemato-philic to nemato-phobic. Additionally, we observe that PEG-doped nematic SSY retains the double-twist director configuration as in the PEG-free case. However, the PEG-doped nematic SSY is accompanied by unprecedented domain-wall-like defects and heterogeneity in the director configuration. We propose multiple hypotheses on how PEG changes the director configuration, including the formation of meta-stable director configurations.

Circulating tumor cells (CTCs) in the blood have been used as diagnostic markers in patients with colorectal cancer (CRC). In this study, we evaluated a CTC detection system based on cell size to assess CTCs and their potential as early diagnostic and prognostic biomarkers for CRC

The challenge lies in understanding the recruitment of immune cells to the tumor site with the contradictory outcomes: tumor reduction or progression. The movement of immature dendritic cells, which navigate and sample the environment before activating the immune response, is random by nature, and recognizing the effective chemotactic cues towards cancer among a myriad of cytokines present in the surroundings is finding a needle in a haystack. In this paper, we find imposing physical constraints in the cell migration tracks in the geometry and dimensions modulates the directional persistence of dendritic cells. A delicate balance between chemotactic cues and the physical confinements reveals subtle chemotactic differences of dendritic cells in cancer vs. normal cell surroundings even inside a complex maze.

Herein, we report a cell-membrane-modified field effect transistor (FET) as a function-based nanosensor for the detection and quantitative measurement of numerous toxins and biological samples. By coating carbon nanotube FETs with natural red blood cell membranes, the resulting biomimetic nanosensor can selectively interact with and absorb broad-spectrum hemolytic toxins regardless of their molecular structures. Toxin–biomembrane interactions alter the local charge distribution at the FET surface in an ultrasensitive and concentration-dependent manner, resulting in a detection limit down to the femtomolar (fM) range.

Vijaya Sunkara‡, Chi-Ju Kim‡, Juhee Park, Hyun-Kyung Woo, Dongyoung Kim, Hong Koo Ha, Mi-Hyun Kim, Youlim Son, Jae-Ryong Kim, Yoon-Kyoung Cho*

Lab-on-a-disc equipped with sequential nanofiltration is presented for fully automated, rapid, label-free EV enrichment with high yield and purity starting from whole blood or plasma for cancer diagnosis and monitoring.

We demonstrate a method to prepare giant unilamellar vesicles (GUVs) with biologically-active protein activity, by mixing erythrocyte (red blood cell) membrane extract with phospholipids and growing their mixture in a porous hydrogel matrix. This presents a pathway to retain protein biological activity without prior isolation and purification of the protein.

This HRP-catalyzed polymerization of TA and p(TA)-mediated surface modification method can provide a simple and new framework to construct multifunctional platforms for covalent attachment of biomolecules and development of sensitive electrochemical sensing devices

Amit Kumar, Sumit Kumar, Nitee Kumari, Seon Hee Lee, Jay Han, Issac Michael, Yoon-Kyoung Cho*, In Su Lee*

Artificial nanoreactors that can facilitate catalysis in living systems on-demand with the aid of remotely operable and biocompatible energy-source, are needed to leverage the chemical diversity and expediency of advanced chemical synthesis in biology and medicine. Here, we designed and synthesized plasmonically-integrated nanoreactors (PINERs) with highly tunable structure and NIR-light-induced synergistic function for efficiently promoting unnatural catalytic reactions inside living cells.

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Androgen receptor splice variant 7 (AR-V7) is associated with castration-resistant prostate cancer (CRPC) and resistance to anti-androgen therapy. Herein, we suggest a practical and non-invasive liquid biopsy method for analysis of AR-V7 in the RNA of urine-derived extracellular vesicles (EVs) without the need for blood withdrawal. Higher AR-V7 and lower AR-FL expression were detected in urine-derived EVs from 14 patients with CRPC than in those from 22 patients with hormone-sensitive prostate cancer. This study is the first to report that RNA of urine-derived EVs is a reliable source for AR-V7 expression analysis. The proposed method for quantifying AR-V7 in urinary EVs prepared by a lab-on-a-disc is, therefore, a simple and promising approach to liquid biopsy with great potential for therapeutic impact on prostate cancer.


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